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德乐NBB-PCR培养基
富集啤酒腐败菌用于后续 PCR 分析
分子生物学快速检测方法，例如 PCR 分析（聚合酶链式反应），是对传统培养方法（例如用于识别阳性结果的培养方法）的 补充。
由于其高度敏感，PCR 还可用于分析啤酒样品，例如在需要快速结果的采样点。 PCR 设备只能处理仅几微升的微小样品量。为了进行检测，必须提前 1-2 (3) 天对培养物进行富集。
NBB®-PCR Broth 专为标准化预富集和后续 PCR 检测而开发，不含啤酒腐败细菌 DNA 和 PCR 抑制剂。特定的营养成分可促进梳齿菌等严格厌氧细菌的生长。和巨型球菌属。除了乳酸菌之外，从而使基于 PCR 的检测能够在大约 48 小时后成功进行。样品谱包括（冲洗）水、酵母和啤酒样品。
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NBB® PCR Broth is a sterile, ready-to-use broth (pH 5.7 ± 0.2) for the standardized and selectiveenrichment of beer spoiling bacteria and their subsequent PCR-based detection. These includeLactobacillus brevis, Lactobacillus lindneri and Pediococus damnosus which account for approx.70-80 % of the contaminations in the brewing environment. But also strictly anaerobic species ofPectinatus or Megasphaera are responsible as obligate beer spoiling bacteria for 3-7 % of thecontamination events. Although molecular PCR-based methods provides detection and identification ofthese bacteria within few hours, cultural pre-enrichment is necessary for trace detection. Thispre-enrichment determines the total detection speed of PCR-based methods, which can take up toseveral days for slowly growing beer spoiling bacteria.The optimized ratio of growth and inhibitory substances of NBB®-PCR Broth offers optimal selectivityand favors not only lactic acid bacteria but also strictly anaerobic Pectinatus spp. andMegasphaera spp. in their growth. Therefore, successful PCR detection down to species level is alreadypossible after 48-72 h of cultural pre-enrichment. NBB®-PCR Broth is universally suitable for allbrewery samples such as yeast, turbid/unfiltered beers, filtered beers and water samples. In addition,through a specially adapted production process, NBB®-PCR Broth is free of DNA and PCR inhibitors andcan be applied with all market-standard PCR kits.